Loading…

Protocol - Gut and Pancreatic Peptides

Add to My Toolkit
Description:

This protocol provides instructions for drawing, processing, and storing blood from the National Health and Nutrition Examination Survey (NHANES) 2007-2008 protocol.

Analysis of the following gut and pancreatic peptides is recommended: amylin, ghrelin, PPY (3-36), PPY (total), GIP (total), GPL-1 (active), and pancreatic polypeptide PP.

Toolkit users are referred to the U.S. Food and Drug Administration’s (FDA’s) approved laboratory assays.

Protocol:

The following is a summary version of the full National Health and Nutrition Examination Survey (NHANES) 2007-2008 protocol.

Exclusion Criteria

Persons will be excluded from this component if they:

  • report that they have hemophilia, or
  • report that they have received cancer chemotherapy in the last 4 weeks.

SP = Sample Person

1. Do you have hemophilia?

1 [ ] Yes

2 [ ] No

7 [ ] Refused

9 [ ] Don’t Know

If the SP answers "Yes," the SP is excluded from the blood draw.

If the SP answers "No" or "Don’t Know," blood is drawn from the SP.

2. Have you received cancer chemotherapy in the past 4 weeks, or do you anticipate such therapy in the next 4 weeks?

1 [ ] Yes

2 [ ] No

7 [ ] Refused

9 [ ] Don’t Know

If the SP answers "Yes," the SP is excluded from the blood draw.

If the SP answers "No" or "Don’t Know," blood is drawn from the SP.

Note from the Obesity/Adiposity Working Group (WG): The investigator should record the reason a person is excluded from the blood draw.

Venipuncture Procedures

Editor’s Note: Please review chapter 4 of the Laboratory Procedures Manual from the NHANES for a full description of phlebotomy procedures: 2007-2008 NHANES Lab Manual.

Venipuncture should generally be performed using the median cubital, cephalic, or basilica veins in the left arm unless this arm is unsuitable. If the veins in the left arm are unsuitable, look for suitable veins on the right arm. If the veins in the antecubital space on both arms are not suitable, then look for veins in the forearm or dorsal side of the hand on the left arm/hand and then right arm/hand.

Recording the Results of the Venipuncture Procedure

Immediately after completing the venipuncture, record the results of the blood draw, the reasons for a tube not being drawn according to the protocol, and any comments about the venipuncture.

Note from the Obesity/Adiposity WG: The investigator should record whether the blood was drawn and whether the full amount was obtained.

Process the Sample for the Serum

Editor’s Note: Please review chapter 8 of the Laboratory Procedures Manual from the NHANES 2007-2008 for a full description of blood processing procedures: 2007-2008 NHANES Lab Manual. http://www.cdc.gov/nchs/nhanes/nhanes2007-2008/manuals07_08.htm

  • Allow the blood to clot by setting aside for 30-45 minutes at room temperature. Do not clot for more than 1 hour.
  • Centrifuge the tube at room temperature to separate the serum and aliquot into an appropriate storage tube.
  • Determine if the serum is hemolyzed, turbid, lipemic, or icteric. If so, enter a comment to describe the plasma

Laboratory Assay for Gut and Pancreatic Peptides

There are now many laboratory assays that have been approved by the U.S. Food and Drug Administration (FDA). Once an assay is chosen for a particular study, the Working Group recommends that consistent methods are followed so that assay results will be comparable.

Protocol Name from Source:

This section will be completed when reviewed by an Expert Review Panel.

Availability:

Publicly available

Personnel and Training Required

Certified phlebotomist

Laboratory capable of performing required assays

Equipment Needs

Phlebotomy supplies

Requirements
Requirement CategoryRequired
Major equipment No
Specialized training No
Specialized requirements for biospecimen collection Yes
Average time of greater than 15 minutes in an unaffected individual No
Mode of Administration

Bioassay

Life Stage:

Infant, Toddler, Child, Adolescent, Adult, Senior, Pregnancy

Participants:

All ages

Specific Instructions:

To aid in comparability, the Obesity Working Group recommends that the investigator record the make and manufacturer of equipment used and the repeatability and coefficients of variation for the assay.

Always note the state of the blood sample as either fasting or fed.

Selection Rationale

These gut and pancreatic peptides are key clinical indicators, and commercial analysis is readily available. The National Health and Nutrition Examination Survey (NHANES) 2007-2008 protocol was selected as the best standardized methodology for blood collection, processing, and storage. Many commercial companies supply enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits and analysis services.

Language

English

Standards
StandardNameIDSource
Common Data Elements (CDE) Obesity Gastrointestinal Tract and Pancreatic Peptide Assay Value 4995474 CDE Browser
Process and Review

This section will be completed when reviewed by an Expert Review Panel.

Source

Centers for Disease Control and Prevention (CDC). National Center for Health Statistics (NCHS) (2007). National Health and Nutrition Examination Survey Questionnaire. Laboratory Procedures Manual. Hyattsville, MD: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention.

General References

None

Protocol ID:

231001

Variables:
Export Variables
Variable NameVariable IDVariable DescriptionVersiondbGaP Mapping
Research Domain Information
Measure Name:

Gut and Pancreatic Peptides

Release Date:

September 9, 2015

Definition

This is a bioassay to measure gut and pancreatic peptides.

Purpose

Gut and pancreatic peptides are indicators of various factors related to obesity such as glucose homeostasis and gastric motility, physiological regulation of appetite and food intake, and stimulation of appetite and gastric emptying.

Keywords

Obesity, growth hormones, appetite regulation, appetite suppression, gut hormones, National Health and Nutrition Examination Survey, NHANES, peptide, pancreatic, gut